| dc.contributor.author |
Murungi LM, Kimathi RK, Tuju J, Kamuyu G, Osier FHA |
|
| dc.date.accessioned |
2024-08-09T08:56:59Z |
|
| dc.date.available |
2024-08-09T08:56:59Z |
|
| dc.date.issued |
2019-07 |
|
| dc.identifier.uri |
https://doi.org/10.1007/978-1-4939-9550-9_6 |
|
| dc.identifier.uri |
http://repository.kemri.go.ke:8080/xmlui/handle/123456789/885 |
|
| dc.description.abstract |
The enzyme-linked immunosorbent assay (ELISA) is a reliable and relatively low-cost method for measuring soluble ligands such as antibodies and proteins in biological samples. For analysis of specific antibodies in serum, a capture antigen is immobilized onto a solid polystyrene surface from which it can capture the antibodies. The captured antibodies are subsequently detected using a secondary antibody conjugated to an enzyme. Detection is accomplished by addition of a colorimetric substrate, and the readout is absorbance (optical density). Here, we provide a detailed standardized ELISA protocol for the quantification of antibodies against malaria antigens. |
en_US |
| dc.language.iso |
en |
en_US |
| dc.publisher |
Springer Link |
en_US |
| dc.title |
Serological Profiling for Malaria Surveillance Using a Standard ELISA Protocol |
en_US |
| dc.type |
Article |
en_US |