dc.description.abstract |
Brucellosis is a re-emerging zoonotic disease that causes more than half a million
infections to humans every year. The disease is common in most developing
countries although its prevalence often remains unreported due to low suspicion
index by health workers and insufficient capacity to correctly diagnose the disease in
humans. Rapid serologic kits are commonly used in human brucellosis diagnosis
resulting to doubtful data due to false positives and negatives and thus little influence
on policies in brucellosis control efforts.
In Sub-Saharan Africa, approximately 16% of livestock habour the disease yet its
treatment in animals is not recommended. Instead animals should be culled and this
practice is not possible in the developing countries due to economic implications and
poor compensation rates by the governments. This therefore has resulted to
endemicity of the disease and continued source of infection to humans.
Although human mortality due to brucellosis is only about 2%, the disease causes
severe disabling sequele like rheumatism, infertility in males, spontaneous abortion
and also results to wastage of resources through prolonged treatment, up to six
weeks, and loss of income through loss of working hours. Brucella organisms are
also considered potential biological weapon which could be cheaper to produce but
more devastating than chemical weapons.
Even though animal brucellosis cases have been reported from Ijara District,
prevalence of the disease in humans is unknown and the associated factors as well as
the effectiveness of the Febrile Rapid Diagnostic Kit®
have not been determined.
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This hospital based cross sectional study was therefore carried out between
December 2010 and January 2011 among 384 febrile patients aged 2 years and
above with the objectives of determining the prevalence and factors associated with
brucellosis and also evaluation of the Febrile Rapid Diagnostic Kit®
used at the
facility.
About 5 mililitres of blood from each patient was drawn and analyzed by the
Febrile Rapid Diagnostic Kit®
and Polymerase chain reaction (PCR). Semi
structured questionnaire administered to collect data. Epi- info version 3.5.1 was
used for data analysis.
Seroprevalence of brucellosis was 31.8% and the true prevalence was 15.4% by
PCR. Obtaining milk from the market (p value <0.00001, odds ratio 7.3, 95%
confidence interval 2.5-21.1) and drinking of unboiled milk (p value <0.0001, odds
ratio 8.5, 95% confidence interval, 4.2-17.3) were significantly associated with
brucellosis. The sensitivity and specificity of the Febrile Rapid Diagnostic Kit® was
37 % and 69% respectively with a Predictive value positive of 18% and Predictive
value negative of 86%. The level of agreement was 0.03.
The findings obtained from this study indicate that brucellosis is prevalent in about
1/6 of febrile patients attending Ijara District Hospital. Unprocessed milk from the
market and consumption of unboiled milk were associated with brucellosis. Febrile
Diagnostic rapid kit®
underestimates positivity but overestimates prevalence of
brucellosis in the febrile patients
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Therefore, patients with brucellosis should be treated to prevent the devastating
effect of the disease and the accompanying sequelae, public health education
programs should explain modes of transmission (milk should be boiled before
consumption) and Febrile Rapid Diagnostic kit®
used at the facility should be
replaced with better rapid diagnostic tests or PCR. |
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