Abstract:
Tuberculosis (TB) is yet far from being controlled. Despite the fact that several
reasons could be attributed to this, a significant contributing factor is the development of
resistance to the currently available drugs due to the successful adaptation of the pathogen to these drugs. Second-line anti-TB drugs are being used for treatment of Multi-Drug Resistant TB (MDR-TB) patients.
The purpose of this study was to investigate the presence of drug resistant strains of
Mycobacterium tuberculosis (MTB) to second-line anti-TB drugs (SLDs) in first-line
predetermined drug susceptibility isolates obtained from different studies carried out at the
Centre for Respiratory Diseases Research (CRDR) between 2002 and 2007.
A total of 216 MTB isolates including 78 first-line drug resistant isolates to
individual and combined drugs and 138 first-line drugs susceptible isolates to all drugs
were selected for this study. Of the 78 first-line resistant isolates, 25 isolates were MDRTB strains. Resistant ratio and proportion methods were used to test. All the isolates were
tested for susceptibility to four second-line drugs including cycloserine, gatifloxacin,
ethionamide and kanamycin. Using S.P.S.S. computer data analysis programme, analysis
of data was done using chi-square to compare resistance and susceptibility among the
drugs, and to compare resistance and susceptibility between the first-line susceptible and
resistant isolates to second-line anti-TB drugs.
Of the 216 first-line isolates tested, 96.3% were sensitive, 2.2% were fully resistant
and 1.5% had intermediate resistance. Of the 78 isolates tested, 94.9% were sensitive, 4.2% were fully resistant and 1% were intermediate resistant. Of the 138 isolates tested, 97.1% were sensitive, 1.1% were fully resistant and 1.8% were intermediate resistant. Drug resistance to second-line anti-TB drugs was not statistically associated with previous firstline anti-TB drugs resistance, although the resistance level of second-line anti-TB drugs in the first-line resistant isolates was higher than in the first-line sensitive isolates.
Resistance of MTB to second-line anti-TB drugs is present in Kenya. There was
resistance to all the four second-line anti-TB drugs tested in this study, in both first-line
resistant and sensitive isolates. There was no XDR-TB strain isolated.
As resistant MTB increases in Kenya further studies are needed to evaluate secondline DST techniques and establish an appropriate one within the national policies. Since the
samples used in this study may not be a representation of the current national status of drug resistance to second-line anti-TB drugs, a national surveillance is important to establish the prevalence of second-line anti-TB drugs resistance in the country