Abstract:
Background: Poliomyelitis is an acute viral infection caused by poliovirus and transmitted via the fecal–oral route.
The causative agent is one of the three serotypes of poliovirus (serotypes 1, 2, 3) that difer slightly in capsid protein.
Prolonged vaccine-related poliovirus shedding in human immunodefciency virus (HIV) positive individuals has been
linked to possible reservoir for reintroduction of polioviruses after eradication. The study therefore aimed at estimating
the duration for vaccine-related poliovirus shedding among potentially and HIV-infected persons.
Methods: Poliovirus excretion was studied following vaccination of children aged ≤ 59 month per human immunodefciency virus status after national immunization days. Their medical records were reviewed to identify the child’s
HIV status, demographic and immunization data. Sequential stool samples were collected at site 2nd, 4th and 8th
week after trivalent oral poliovirus vaccine (tOPV) was administered. To isolate suspected polioviruses and non-polio
enteroviruses, characterize poliovirus subtypes by intratypic diferentiation and Sabin vaccine derived poliovirus, real
time polymerase chain reaction was applied. Shedding for ≥ 24 weeks was defned as long-term persistence.
Results: The mean age of the study population was 28.6 months, while the median age was 24 months. Of the children recruited, majority were in the 25–48 months (n = 12; 46.2%) age category. All the HIV-positive children (n = 10)
had mild symptomatic HIV status and did shed vaccine-related polioviruses between weeks 2 and 4 respectively. No
participant shed polioviruses for ≥ 6 weeks.
Conclusions: It was evident mildly symptomatic HIV+ children sustain the capacity to clear vaccine-related poliovirus. The oral poliovirus vaccine-2 (Sabin like) that was detected in one HIV-infected child’s stool 6 weeks after the
national immunization days was predominantly non revertant. There was no evident prolonged poliovirus shedding
among the participants enlisted in the present study. High powered studies are desired to further corroborate these
findings.
